| Accession | CRX1279436 |
| Organism | Xenocypris davidi |
| Title | FDHC220003087 |
| BioProject | PRJCA033001 |
| BioSample | SAMC4415729 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
To investigate the three-dimensional architecture of the genome, we first fixed the chromatin conformation of cells using paraformaldehyde. Subsequently, we lysed the fixed cells and treated the crosslinked DNA with restriction Mbo I enzymes to generate sticky ends. We then repaired the DNA ends and simultaneously labeled them with biotin. DNA ligase was used to join the DNA fragments. Finally, we reversed the crosslinking state by protease digestion, purified the DNA, and randomly sheared it into fragments ranging from 300 to 500 base pairs. |
Hi-C |
GENOMIC |
other |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
|
| Release date | 2024-12-10 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR1395561 |
CRR1395561_r1.fq.gz
CRR1395561_r2.fq.gz
|
21,038.92
21,959.47
|
| CRR1395562 |
CRR1395562_r1.fq.gz
CRR1395562_r2.fq.gz
|
3,713.49
3,792.35
|
|
| Submitter | Tiezhu Yang (tzyang@xyafu.edu.cn) |
|---|
| Organization | Xinyang Agriculture and Forestry University |
|---|
| Date submitted | 2024-11-29 |
|---|
