| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
DNA fragmentation was carried out using Oxford Nanopore’s DNA fragmentation method, and adapters were ligated to the DNA fragments. Library quality control was conducted using the Agilent Bioanalyzer to assess fragment size distribution, ensuring the library was suitable for sequencing. Sequencing was performed on the Oxford Nanopore platform using electrophoretic migration to load DNA samples into nanopore chips for real-time single-molecule sequencing. Data acquisition and preliminary processing were carried out using the Oxford Nanopore MinKNOW software, and raw sequencing data were obtained in FASTQ format, from which the full length of 16S rRNA sequence was extracted. |
WGS |
GENOMIC |
size fractionation |
SINGLE
|
|
