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Libraries were constructed by MGIEasy Universal DNA Library Prep Kit V1.0(CAT#1000005250, MGI) following the standard protocol. Briefly, 1ug genomic DNA was randomly fragmented by Covaris. The fragmented DNA was selected by MGIEasy DNA Clean beads (CAT#1000005279, MGI) to an average size of 200-400bp. After that, the selected fragments were end-repaired, 3'adenylated and then ligated adapters. The DNA samples were amplified by PCR and the products were purified by the MGIEasy DNA Clean beads (CAT#1000005279, MGI). The double stranded PCR products were heat denatured and circularized by the splint oligo sequence in MGIEasy Circularization Module (CAT#1000005260, MGI). The single strand circle DNA (ssCir DNA) were formatted as the final library and qualified by QC. The qualified libraries were sequenced on DNBSEQ-T7RS platform. |
WGS |
GENOMIC |
other |
PAIRED
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