| 实验编号 | CRX1313304 |
| 物种名称 | Mus musculus |
| 标题 | KD_5 |
| 项目编号 | PRJCA019436 |
| 样本编号 | SAMC4476411 |
| 测序平台 | Illumina Genome Analyzer |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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This project enriched mRNA with polyA structures from total RNA using Oligo(dT) magnetic beads. RNA was fragmented to approximately 300 bp using ion fragmentation. The selection of 300 bp fragments is critical because the adapter sequences have a fixed length. If the RNA fragments are too short, the proportion of adapter sequences will increase, reducing the effective data yield. Conversely, longer fragments are not conducive to cluster formation during sequencing. Using RNA as a template, the first strand of cDNA was synthesized with random hexamer primers and reverse transcriptase. The second strand of cDNA was subsequently synthesized using the first-strand cDNA as a template. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 300
Planned read length (bp) for mate 2: 300
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| 发布日期 | 2025-05-15 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1429960 |
CRR1429960_r1.fastq.gz
CRR1429960_r2.fastq.gz
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1,830.99
1,850.02
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|
| 提交者 | Lianxin Liu (liulx@ustc.edu.cn) |
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| 所属单位 | The First Affiliated Hospital of University of Science and Technology of China |
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| 提交日期 | 2024-12-21 |
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