| 实验编号 | CRX1319120 |
| 物种名称 | Xanthoceras sorbifolium |
| 标题 | RNA-seq for T1 stage flower of PBN-43 |
| 项目编号 | PRJCA033489 |
| 样本编号 | SAMC4462783 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2025-01-11 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1435986 |
CRR1435986_r1.fq.gz
CRR1435986_r2.fq.gz
|
1,560.66
1,566.35
|
| CRR1435987 |
CRR1435987_r1.fq.gz
CRR1435987_r2.fq.gz
|
1,812.03
1,814.21
|
| CRR1435988 |
CRR1435988_r1.fq.gz
CRR1435988_r2.fq.gz
|
1,700.55
1,694.43
|
|
| 提交者 | Yijun Chen (yijun.chen@pku-iaas.edu.cn) |
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| 所属单位 | Peking University Institute of Advanced Agricultural Science |
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| 提交日期 | 2024-12-25 |
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