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实验基本信息
实验编号 CRX1319123
物种名称 Xanthoceras sorbifolium
标题 RNA-seq for PBN-126 leaf
项目编号 PRJCA033489
样本编号 SAMC4462786
测序平台 Illumina NovaSeq 6000
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
发布日期2025-01-11
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR1435995 CRR1435995_r1.fq.gz
CRR1435995_r2.fq.gz
2,112.12
2,108.8
CRR1435996 CRR1435996_r1.fq.gz
CRR1435996_r2.fq.gz
1,650.75
1,641.98
CRR1435997 CRR1435997_r1.fq.gz
CRR1435997_r2.fq.gz
1,522.24
1,522.09
提交者Yijun Chen (yijun.chen@pku-iaas.edu.cn)
所属单位Peking University Institute of Advanced Agricultural Science
提交日期2024-12-25