| 实验编号 | CRX1327216 |
| 物种名称 | Mus musculus |
| 标题 | ctrl5 |
| 项目编号 | PRJCA034574 |
| 样本编号 | SAMC4527412 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
The eukaryotic mRNA with polyA tails is enriched using magnetic beads with Oligo(dT) and then fragmented using a buffer. The fragmented mRNA serves as a template to synthesize the first strand of cDNA using random oligonucleotides as primers in an M-MuLV reverse transcriptase system. The RNA strand is then degraded using RNaseH, and the second strand of cDNA is synthesized using DNA polymerase I with dNTPs as raw materials. The purified double-stranded cDNA undergoes end repair, addition of an A-tail, and ligation with sequencing adapters. AMPure XP beads are used to select cDNA fragments of approximately 200 bp, followed by PCR amplification. The PCR products are then purified again using AMPure XP beads to generate the final sequencing library. |
RNA-Seq |
TRANSCRIPTOMIC |
unspecified |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2025-01-13 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1444703 |
CRR1444703_r1.fq.gz
CRR1444703_r2.fq.gz
|
1,678.37
1,848.67
|
|
| 提交者 | Yisi Liu (1004371737@qq.com) |
|---|
| 所属单位 | Southern Medical University, Zhujiang hospital |
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| 提交日期 | 2025-01-05 |
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