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实验基本信息
实验编号 CRX1327403
物种名称 mouse gut metagenome
标题 CV53
项目编号 PRJCA034574
样本编号 SAMC4527993
测序平台 Illumina MiSeq
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Bacterial genomic DNA was isolated utilizing the MinkaGene Stool DNA Kit in accordance with the guidelines provided by the manufacturer. Amplification of the V4 variable region of the 16S rRNA gene was achieved using barcoded primers V4F (GTGYCAGCMGCCGCGGTAA) and V4R (GGACTACNVGGGTWTCTAAT). PCR was conducted as previously outlined. Subsequently, all PCR products were pooled and sequenced on an Illumina iSeq 100 system following the manufacturer's instructions. AMPLICON GENOMIC PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
发布日期2025-01-10
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR1444893 CRR1444893_r1.fq.gz
CRR1444893_r2.fq.gz
2.97
2.31
提交者Yisi Liu (1004371737@qq.com)
所属单位Southern Medical University, Zhujiang hospital
提交日期2025-01-05