| 实验编号 | CRX1327403 |
| 物种名称 | mouse gut metagenome |
| 标题 | CV53 |
| 项目编号 | PRJCA034574 |
| 样本编号 | SAMC4527993 |
| 测序平台 | Illumina MiSeq |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Bacterial genomic DNA was isolated utilizing the MinkaGene Stool DNA Kit in accordance with the guidelines provided by the manufacturer. Amplification of the V4 variable region of the 16S rRNA gene was achieved using barcoded primers V4F (GTGYCAGCMGCCGCGGTAA) and V4R (GGACTACNVGGGTWTCTAAT). PCR was conducted as previously outlined. Subsequently, all PCR products were pooled and sequenced on an Illumina iSeq 100 system following the manufacturer's instructions. |
AMPLICON |
GENOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2025-01-10 |
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
| CRR1444893 |
CRR1444893_r1.fq.gz
CRR1444893_r2.fq.gz
|
2.97
2.31
|
|
| 提交者 | Yisi Liu (1004371737@qq.com) |
| 所属单位 | Southern Medical University, Zhujiang hospital |
| 提交日期 | 2025-01-05 |