Accession | CRX1327422 |
Organism | mouse gut metagenome |
Title | H176 |
BioProject | PRJCA034574 |
BioSample | SAMC4528012 |
Platform | Illumina MiSeq |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Bacterial genomic DNA was isolated utilizing the MinkaGene Stool DNA Kit in accordance with the guidelines provided by the manufacturer. Amplification of the V4 variable region of the 16S rRNA gene was achieved using barcoded primers V4F (GTGYCAGCMGCCGCGGTAA) and V4R (GGACTACNVGGGTWTCTAAT). PCR was conducted as previously outlined. Subsequently, all PCR products were pooled and sequenced on an Illumina iSeq 100 system following the manufacturer's instructions. |
AMPLICON |
GENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
Release date | 2025-01-10 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR1444912 |
CRR1444912_r1.fq.gz
CRR1444912_r2.fq.gz
|
5.74
4.7
|
|
Submitter | Yisi Liu (1004371737@qq.com) |
Organization | Southern Medical University, Zhujiang hospital |
Date submitted | 2025-01-05 |