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Experiment information
Accession CRX1327427
Organism mouse gut metagenome
Title HT12
BioProject PRJCA034574
BioSample SAMC4528017
Platform Illumina MiSeq
Library
Library name Construction protocol Strategy Source Selection Layout
Bacterial genomic DNA was isolated utilizing the MinkaGene Stool DNA Kit in accordance with the guidelines provided by the manufacturer. Amplification of the V4 variable region of the 16S rRNA gene was achieved using barcoded primers V4F (GTGYCAGCMGCCGCGGTAA) and V4R (GGACTACNVGGGTWTCTAAT). PCR was conducted as previously outlined. Subsequently, all PCR products were pooled and sequenced on an Illumina iSeq 100 system following the manufacturer's instructions. AMPLICON GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2025-01-10
Run
Run accession Run data file information
File nameFile size (MB)
CRR1444917 CRR1444917_r1.fq.gz
CRR1444917_r2.fq.gz
6.32
5.21
SubmitterYisi Liu (1004371737@qq.com)
OrganizationSouthern Medical University, Zhujiang hospital
Date submitted2025-01-05