| 实验编号 | CRX1331576 |
| 物种名称 | respiratory tract metagenome |
| 标题 | MBX199135 |
| 项目编号 | PRJCA034623 |
| 样本编号 | SAMC4528841 |
| 测序平台 | MGISEQ-2000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Biological specimens including bronchoalveolar lavage fluid (BALF) or sputum, were collected from enrolled patients suspected of having tuberculosis. A minimum of 800 μL of each sample was obtained. Microbial DNA was extracted using nucleic acid extraction and purification reagents from GenK magnetic nead-based DNA/RNA extraction kit. Extracted DNA was subjected to targeted multiplex-PCR using a pre-designed primer panel (covering targeted regions of 64 microbial species and 158 drug resistant sites of MTB) to enrich pathogen-specific genomic fragments. This step utilized the GenK Tuberculosis Infection Pathogen Nucleic Acid Detection Kit (Multiple amplification method). After the targeted enrichment, adapters were added to the DNA to complete the library construction. |
OTHER |
METAGENOMIC |
PCR |
SINGLE
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| 处理信息 |
Planned read length (bp): 50
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| 发布日期 | 2025-10-05 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
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| CRR1449080 |
CRR1449080.fq.gz
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113.89
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| 提交者 | Fengshuo Sun (sunfs12345@126.com) |
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| 所属单位 | Genskey |
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| 提交日期 | 2025-01-07 |
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