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实验基本信息
实验编号 CRX1334067
物种名称 Orchidaceae
标题 Gastrodia elata_C_S9
项目编号 PRJCA034717
样本编号 SAMC4534273
测序平台 Illumina NovaSeq 6000
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
After the total RNA extracted from the sample was qualified, the eukaryotic mRNA was enriched by magnetic beads connected with Oligo(dT). The extracted mRNA was divided into fragments randomly by Fragmentation Buffer. A cDNA strand was synthesized using six-base Random hexamers based on the fragment mRNA template. Buffer solution, dNTPs, RNaseH and DNA Polymerase I were then added to synthesize two-strand cDNA. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
发布日期2025-01-10
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR1451626 CRR1451626_r1.fastq.gz
CRR1451626_r2.fastq.gz
1,125.88
1,204.5
提交者jie Liu (liujie6470@163.com)
所属单位China Three Gorges University
提交日期2025-01-08