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实验基本信息
实验编号 CRX1476787
物种名称 Mus musculus
标题 Ribo_WT_1
项目编号 PRJCA035549
样本编号 SAMC4646932
测序平台 Illumina NovaSeq 6000
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Cells were treated with 100 μg/mL cycloheximide, lysed and centrifuged to collect the supernatant, which was then nuclease digested with RNase I and DNase I to obtain the ribosome-protected fragments (RPFs). RPFs were separated by the RNA Clean and Concentrator-28 kit and purified by magnet beads. The library was constructed using NEBNext Multiple Small RNA Library Prep Set for Illumina Ribo-seq TRANSCRIPTOMIC other PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
发布日期2026-03-10
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR1597633 CRR1597633_r1.fq.gz
CRR1597633_r2.fq.gz
2,388.18
2,193.73
提交者Quan Yuan (yuanquan@scu.edu.cn)
所属单位West China Hospital of Stomatology, Sichuan University
提交日期2025-01-24