| 实验编号 | CRX1476789 |
| 物种名称 | Mus musculus |
| 标题 | Ribo_KO_1 |
| 项目编号 | PRJCA035549 |
| 样本编号 | SAMC4646934 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Cells were treated with 100 μg/mL cycloheximide, lysed and centrifuged to collect the supernatant, which was then nuclease digested with RNase I and DNase I to obtain the ribosome-protected fragments (RPFs). RPFs were separated by the RNA Clean and Concentrator-28 kit and purified by magnet beads. The library was constructed using NEBNext Multiple Small RNA Library Prep Set for Illumina |
Ribo-seq |
TRANSCRIPTOMIC |
other |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2026-03-10 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1597635 |
CRR1597635_r1.fq.gz
CRR1597635_r2.fq.gz
|
3,940.28
3,522.11
|
|
| 提交者 | Quan Yuan (yuanquan@scu.edu.cn) |
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| 所属单位 | West China Hospital of Stomatology, Sichuan University |
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| 提交日期 | 2025-01-24 |
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