| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Using RNA as template, the first strand of cDNA was synthesized with 6-base random primers and reverse transcriptase, and The second strand cDNA was synthesized using the first strand cDNA as template. Using RNA as template, the first strand of cDNA was synthesized with 6-base random primers and reverse transcriptase, and The second strand cDNA was synthesized using the first strand cDNA as template. After the library construction, PCR amplification was used to enrich the library fragments, and then the library was selected according to the fragment size. After the library construction was completed, PCR amplification was used to enrich the library fragments, and then the library was selected according to the fragment size, and the library size was 450bp |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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