| 实验编号 | CRX1547702 |
| 物种名称 | Mus musculus |
| 标题 | Butyrate3 |
| 项目编号 | PRJCA036993 |
| 样本编号 | SAMC4815828 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Extract RNA from small intestinal IECs by TRIZOL. Assess RNA quality using NanoDrop 2000 and Agilent Bioanalyzer 2100. Create sequencing libraries with Hieff NGS kit, add indexes, and sequence on Illumina NovaSeq. Calculate gene expression by FPKM. Do differential analysis with DESeq2_EBSeq (FDR < 0.05, FC ≥ 1.5). Visualize via volcano plot. Analyze KEGG pathway enrichment of differential genes using KOBAS and clusterProfiler. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2025-03-07 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1672512 |
CRR1672512_r1.fq.gz
CRR1672512_r2.fq.gz
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1,277.67
1,391.38
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| 提交者 | Jialu Shi (sjl951130@163.com) |
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| 所属单位 | Southeast University |
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| 提交日期 | 2025-03-07 |
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