| 实验编号 | CRX1561183 |
| 物种名称 | Sinocyclocheilus microphthalmus |
| 标题 | ZZP-03 |
| 项目编号 | PRJCA037331 |
| 样本编号 | SAMC4860185 |
| 测序平台 | Illumina HiSeq 2000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
(1) Eukaryotic mRNA was enriched with magnetic beads with Oligo (dT); (2) mRNA was randomly interrupted by the addition of Fragmentation Buffer; (3) The first cDNA strand was synthesised using mRNA as a template with random hexamers (six-base random primers), followed by the addition of buffers, dNTPs, RNase H and DNA polymerase I to synthesise the second cDNA strand, and the cDNA was purified using AMPure XP beads; (4) the purified double-stranded cDNA was then subjected to end repair, addition of A-tail and ligation of sequencing junctions, and then fragment size selection was performed using AMPure XP beads; and (5) finally, cDNA library was obtained by enrichment via PCR The cDNA library was then enriched by PCR. |
WGS |
GENOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2025-08-18 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1687764 |
CRR1687764_r1.fq.gz
CRR1687764_r2.fq.gz
|
6,708.86
6,837.37
|
|
| 提交者 | Min Wang (wangmin2019@ioz.ac.cn) |
|---|
| 所属单位 | Institute of Zoology, Chinese Academy of Sciences |
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| 提交日期 | 2025-03-17 |
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