|
Cells were loaded onto the 10X Chromium Single Cell Platform (10X Genomics) at a concentration of 1,000 cells per μl (Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) ) as described in the manufacturer’s protocol. Generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, complementary DNA amplification and library construction were all performed as per the manufacturer’s protocol. Qubit was used for library quantification before pooling. The final library pool was sequenced on the Illumina Novaseq X plus instrument using 150-base-pair paired-end reads. |
OTHER |
TRANSCRIPTOMIC SINGLE CELL |
PolyA |
PAIRED
|
