| 实验编号 | CRX1591586 |
| 物种名称 | respiratory tract metagenome |
| 标题 | ET796 |
| 项目编号 | PRJCA037984 |
| 样本编号 | SAMC4933762 |
| 测序平台 | NextSeq CN500 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Nucleic acid extraction was performed using a column-based method in which functional groups with an affinity for nucleic acids were fixed onto a centrifugal column. By adding different lysis and washing reagents followed by repeated centrifugation, nucleic acids were separated from impurities, resulting in purified nucleic acids. The library was then constructed using a specialized pathogen DNA library preparation kit. In this process, transposase was used to fragment the genome into appropriately sized segments. Sequencing adapters compatible with the Illumina™ sequencing platform were attached to both ends of the fragments, and specific regions were captured using custom or specific primers. Subsequently, PCR amplification was performed to increase the library quantity to meet sequencing requirements. |
WGS |
METAGENOMIC |
other |
SINGLE
|
|
| 处理信息 |
Planned read length (bp): 50
|
| 发布日期 | 2025-04-04 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1718959 |
CRR1718959.fastq.gz
|
492.15
|
|
| 提交者 | Xiuyu Xu (xuxiuyu85@126.com) |
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| 所属单位 | The First Affiliated Hospital of Chongqing Medical University |
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| 提交日期 | 2025-04-03 |
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