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We collected gonadal tissues from male and female Bb individuals for ATAC-seq analysis, with each group comprising two biological replicates. The collected samples were washed with 0.04% BSA in 3x PBS and then lysed on ice for 7 minutes using a lysis buffer containing 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, 3 mM MgCl2, 0.5% NP-40, and 0.1% Tween 20. During lysis, gentle pipetting was performed until the tissues were completely disrupted, releasing the nuclei. The nuclei were then washed and resuspended in a pre-cooled wash buffer (10 mM Tris-HCl, pH 7.5; 10 mM NaCl; 3 mM MgCl2; 0.1% Tween 20) to obtain a nuclear suspension. Subsequently, library construction was carried out according to the manufacturer's instructions using the Chromatin Profile Kit for Illumina (Novoprotein, N248). |
ATAC-seq |
GENOMIC |
other |
PAIRED
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