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Qualified genomic DNA samples were randomly broken into fragments of about 250 bp. Then the DNA sample was end-repaired and ligated to adapters, followed by purification with magnetic beads and PCR amplification using KAPA HiFi HotStart DNA Polymerase. Denature the library to single-strand DNA, circularize, digest linear DNA, and quantify the circularized library using a Qubit Fluorometer (Thermo Scientific, MA, USA). The qualified DNA library was sequenced by IGENEBOOK company (Wuhan, China) using the DNBSEQ-T7 platform. |
WGS |
GENOMIC |
PCR |
PAIRED
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