| 实验编号 | CRX1772237 |
| 物种名称 | Mus musculus |
| 标题 | mb_dim1-1 |
| 项目编号 | PRJCA040561 |
| 样本编号 | SAMC5123722 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Total RNA from mouse brain was depleted of rRNA using thermostable RNase H and DNase-treated oligo pools, followed by magnetic bead purification. To avoid 3'-end bias, poly(A) tails were enzymatically removed prior to zinc-mediated fragmentation. The resulting RNA fragments were end-repaired with T4 PNK and ligated to a biotinylated 3' adapter. After enzymatic digestion of excess adapters, ligated RNA was captured on streptavidin beads. Experimental samples (n = 2) were subjected to two rounds of allyl labeling using MjDim1 and allyl-SAM, followed by iodine-induced cyclization at modified residues. Control samples (n = 2) were processed in parallel but skipped the labeling step entirely. All samples underwent reverse transcription using actinomycin D-supplemented HIV reverse transcriptase to generate mutation-encoded cDNA. A second adapter was ligated to the 3' end of the cDNA using T4 RNA ligase 1 in a high-PEG and cobalt hexammine buffer system. All enzymatic steps were followed by magnetic bead purification, and libraries were stored at -20 C for downstream PCR and sequencing. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2025-06-13 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
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| CRR1905250 |
CRR1905250_r1.fastq.gz
CRR1905250_r2.fastq.gz
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4,360.23
4,827.1
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| 提交者 | Han Dong (donghan@biols.ac.cn) |
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| 所属单位 | Institute of Zoology, Chinese Academy of Sciences |
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| 提交日期 | 2025-06-11 |
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