| 实验编号 | CRX1772239 |
| 物种名称 | Mus musculus |
| 标题 | mb_ONT |
| 项目编号 | PRJCA040561 |
| 样本编号 | SAMC5123724 |
| 测序平台 | OXFORD_NANOPORE MinION |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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The RT Adaptor (RTA) was ligated to the 3' end of Poly(A)+ RNA by T4 DNA ligase and then reverse transcribed by SuperScript III Reverse Transcriptase. The RNA was purified by 1.8x RNAClean XP beads and then the RNA Adaptor was ligated to the 3' end of Poly(A)+ RNA using T4 DNA ligase and then the RNA was purified with 1x RNAClean XP beads. The sample was eluted with Elution Buffer. Then, the sample was loaded onto a R9.4.1 flow cell in a MinION sequencer. Each flow cell was sequenced for 72 h. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
SINGLE
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| 处理信息 |
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| 发布日期 | 2025-06-13 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
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| CRR1905252 |
CRR1905252.fastq.gz
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2,466.69
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| 提交者 | Han Dong (donghan@biols.ac.cn) |
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| 所属单位 | Institute of Zoology, Chinese Academy of Sciences |
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| 提交日期 | 2025-06-11 |
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