| 实验编号 | CRX1779766 |
| 物种名称 | Musculus |
| 标题 | P2X7-KO CD4T-3 |
| 项目编号 | PRJCA041654 |
| 样本编号 | SAMC5311797 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
The purified mRNAs were cleaved into small fragments; First-strand cDNAs were produced by reverse transcription; Second-strand cDNAs are synthesized, while simultaneously performing end repair and dA-Tailing; Sequencing adapter ligation was performed, followed by DNA magnetic bead purification and fragment selection; The ligated products were amplified by PCR and purified again using DNA magnetic beads, where the products were solubilized with nuclear-free water. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2025-08-21 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1913917 |
CRR1913917_r1.fq.gz
CRR1913917_r2.fq.gz
|
1,820.22
1,881.94
|
|
| 提交者 | Wenying Liu (LWYxnyy@foxmail.com) |
|---|
| 所属单位 | Southwest Hospital, Army Medical University |
|---|
| 提交日期 | 2025-06-17 |
|---|
