| 实验编号 | CRX1781813 |
| 物种名称 | Arachis hypogaea |
| 标题 | NH_DS_1 |
| 项目编号 | PRJCA041789 |
| 样本编号 | SAMC5312228 |
| 测序平台 | Illumina NovaSeq 5000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
RNA was extracted using CTAB-pBIOZOL, and mRNA with a polyA tail was enriched using Oligo(dT) magnetic beads; the purified mRNA was fragmented into small pieces; first-strand cDNA was synthesized through reverse transcription using random hexamer primers; second-strand cDNA synthesis was performed, along with end-repair and dA-tailing; sequencing adapter ligation was carried out, followed by DNA magnetic bead purification and fragment selection to obtain a library with insert fragments of 250-350 bp. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2025-12-31 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1915980 |
CRR1915980_r1.fq.gz
CRR1915980_r2.fq.gz
|
1,794.35
1,765.66
|
|
| 提交者 | Ruier Zeng (ruierzeng@126.com) |
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| 所属单位 | South China Agricultural University |
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| 提交日期 | 2025-06-18 |
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