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实验基本信息
实验编号 CRX1781818
物种名称 Arachis hypogaea
标题 SH_DS_3
项目编号 PRJCA041789
样本编号 SAMC5312233
测序平台 Illumina NovaSeq 5000
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
RNA was extracted using CTAB-pBIOZOL, and mRNA with a polyA tail was enriched using Oligo(dT) magnetic beads; the purified mRNA was fragmented into small pieces; first-strand cDNA was synthesized through reverse transcription using random hexamer primers; second-strand cDNA synthesis was performed, along with end-repair and dA-tailing; sequencing adapter ligation was carried out, followed by DNA magnetic bead purification and fragment selection to obtain a library with insert fragments of 250-350 bp. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
发布日期2025-12-31
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR1915985 CRR1915985_r1.fq.gz
CRR1915985_r2.fq.gz
1,845.34
1,817.42
提交者Ruier Zeng (ruierzeng@126.com)
所属单位South China Agricultural University
提交日期2025-06-18