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Prior to conducting Single Molecule Real Time (SMRT) sequencing, high-quality genomic DNA underwent stringent quality control. The genomic DNA was fragmented into suitably sized large fragments, and then damage repair, adapter ligation, and fragment selection were performed to construct a PCR-free SMRT bell library. After size selection and quantification, the SMRT bell library was sequenced on the PacBio Revio platform. Following quality control and filtering using the SMRT Link v12.0 software |
OTHER |
GENOMIC SINGLE CELL |
other |
SINGLE
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