| 实验编号 | CRX1831283 |
| 物种名称 | Oryza sativa |
| 标题 | WGBS-TK24 |
| 项目编号 | PRJCA042637 |
| 样本编号 | SAMC5606626 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Genomic DNA is broken into 100-300 bp fragments by ultrasound. End repair of DNA fragments, addition of A base at the 3 'end, and connection of sequencing adapters. ZYMO EZ DNA Methylation Gold kit was used for Bisulfite treatment. After desalination treatment, the gel was cut and recovered, and the library fragment size was selected. After PCR amplification, the library fragment size was selected again. After the construction of the library is completed, perform quality testing on the library. The library that passes the quality inspection will be used for machine sequencing. |
Bisulfite-Seq |
GENOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
| 发布日期 | 2025-07-17 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1967309 |
CRR1967309_r1.fq.gz
CRR1967309_r2.fq.gz
|
5,068.87
5,198.54
|
|
| 提交者 | Kai Sun (1961168092@qq.com) |
|---|
| 所属单位 | South China Agricultural University |
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| 提交日期 | 2025-07-08 |
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