| 实验编号 | CRX1849200 |
| 物种名称 | mosquito metagenome |
| 标题 | 5M_rep4 |
| 项目编号 | PRJCA042946 |
| 样本编号 | SAMC5624259 |
| 测序平台 | Illumina Nextseq 500 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Total RNA was extracted from mosquitoes using the RNeasy Mini Kit (Qiagen, Germany), following the manufacturer's protocol. First-strand complementary DNA (cDNA) was synthesized via reverse transcription using random hexamer primers and the Illumina TruSeq Stranded Total RNA Kit (Illumina Inc., USA), ensuring comprehensive coverage of the RNA template. Second-strand synthesis was performed using the same kit to generate double-stranded cDNA (ds-cDNA), providing a stable representation of the transcriptome. The resulting ds-cDNA was used for library construction using the Illumina DNA Prep Kit, and indexed libraries were pooled at equimolar concentrations. The pooled libraries were normalized to 4 nM and diluted to a final loading concentration of 1.5 pM before paired-end sequencing on the Illumina NextSeq 550 platform. The resulting high-throughput sequence data served as the basis for metatranscriptomic profiling. |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2025-08-21 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1985415 |
CRR1985415_r1.fastq.gz
CRR1985415_r2.fastq.gz
|
1,085.89
1,086.92
|
|
| 提交者 | Han Xia (hanxia@wh.iov.cn) |
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| 所属单位 | Wuhan Institute of Virology, Chinese Academy of Sciences |
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| 提交日期 | 2025-07-10 |
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