| 实验编号 | CRX1854443 |
| 物种名称 | Arabidopsis thaliana |
| 外部数据库编号 |
: |
| 标题 | AvrRpm1pepr_Root_DEX_rep1 |
| 项目编号 | PRJCA042975 |
| 样本编号 | SAMC5631901 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
The mRNA with a polyA tail is enriched using Oligo(dT) magnetic beads. The obtained mRNA is then randomly fragmented using divalent cations. The fragmented mRNA serves as a template, and random oligonucleotides act as primers to synthesize the first strand of cDNA in the 1st Strand Enzyme reverse transcriptase system. The second strand of cDNA is synthesized using 2nd Strand Enzyme and dNTPs. The purified double-stranded cDNA undergoes end repair, A-tailing, and sequencing adapter ligation. cDNA fragments of 200-300bp are selected, amplified via PCR, and the PCR products are purified again to ultimately obtain the library. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2026-03-03 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1990759 |
CRR1990759_r1.fq.gz
CRR1990759_r2.fq.gz
|
2,716.26
2,183.46
|
|
| 提交者 | Kaixiang Guan (12133029@mail.sustech.edu.cn) |
|---|
| 所属单位 | Southern University of Science and Technology |
|---|
| 提交日期 | 2025-07-14 |
|---|
