| 实验编号 | CRX1868614 |
| 物种名称 | gut metagenome |
| 标题 | Group_HEP_A3 |
| 项目编号 | PRJCA043467 |
| 样本编号 | SAMC5705197 |
| 测序平台 | 454 GS FLX |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Total genomic DNA was extracted using DNA Extraction Kit following the manufacturer’s
instructions. Concentration of DNA was verified with NanoDrop and agarose gel. The genome
DNA was used as template for PCR amplification with the barcoded primers and Tks Gflex DNA
Polymerase (Takara).
For bacterial diversity analysis, V3-V4 (or V4-V5) variable regions of 16S rRNA genes was
amplified with universal primers 343 F and 798 R |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 246
Planned read length (bp) for mate 2: 442
|
| 发布日期 | 2025-07-23 |
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
| CRR2009223 |
CRR2009223_r1.fq.gz
CRR2009223_r2.fq.gz
|
4.29
4.72
|
|
| 提交者 | Cheng Song (lanniao812329218@163.com) |
| 所属单位 | West Anhui University |
| 提交日期 | 2025-07-22 |