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实验基本信息
实验编号 CRX1868614
物种名称 gut metagenome
标题 Group_HEP_A3
项目编号 PRJCA043467
样本编号 SAMC5705197
测序平台 454 GS FLX
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Total genomic DNA was extracted using DNA Extraction Kit following the manufacturer’s instructions. Concentration of DNA was verified with NanoDrop and agarose gel. The genome DNA was used as template for PCR amplification with the barcoded primers and Tks Gflex DNA Polymerase (Takara). For bacterial diversity analysis, V3-V4 (or V4-V5) variable regions of 16S rRNA genes was amplified with universal primers 343 F and 798 R AMPLICON METAGENOMIC PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 246
Planned read length (bp) for mate 2: 442
发布日期2025-07-23
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR2009223 CRR2009223_r1.fq.gz
CRR2009223_r2.fq.gz
4.29
4.72
提交者Cheng Song (lanniao812329218@163.com)
所属单位West Anhui University
提交日期2025-07-22