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Genomic DNA was extracted from muscle using QIAamp DNA Mini Kit/DNeasy Plant Mini Kit 1 (QIAGEN). The integrity of the DNA was determined with the Agilent 4200 Bioanalyzer. Eight micrograms of genomic DNA were sheared using g-Tubes, and concentrated with AMPure PB magnetic beads. Each SMRT bell library was constructed using the Pacific Biosciences SMRT bell template prep kit 1.0. The constructed library was size selected by Sage ELF for molecules 11~15 Kb, followed by primer annealing and the binding of SMRT bell templates to polymerases with the DNA Polymerase Binding Kit. |
Synthetic-long-Read |
GENOMIC |
PCR |
SINGLE
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