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After Sample QC, 500ng of Meta DNA was fragmented by ultrasound on Covaris E220 (Covaris,Brighton, UK), then selected to 300-700 bp by magnet beads size-selection. The selected DNA fragments were repaired, then ligated with indexed adaptor. The ligation product was amplified by PCR, hybridized with exon probe, and captured by streptavidin beads. The Captured DNA was amplified again by PCR, and circularized to get single-stranded circular(ssCir) library.The ssCir library was then amplified through rolling circle amplification (RCA) to obtain DNA nanoball(DNB). The DNB was then loaded to flowcell, and sequenced by DNBSEQ Platform. |
WGS |
METAGENOMIC |
PCR |
PAIRED
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