| 实验编号 | CRX2027201 |
| 物种名称 | moss metagenome |
| 标题 | 22-9 |
| 项目编号 | PRJCA046552 |
| 样本编号 | SAMC5941671 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
A total of 3 ug DNA from each sample was mixed with cesium chloride (CsCl) solution to reach an initial buoyant density of 1.725 g mL-1 in gradient buffer (pH 8.0; 100 mM Tris-HCl, 100 mM KCl, 1.0 mM EDTA). The mixture was transferred to 5.1 mL ultracentrifuge tubes and centrifuged in a vertical rotor at 45000 rpm (190000 x g) for 44 h at 20 C. Following centrifugation, the tubes were removed vertically and fractionated into fifteen 340 uL DNA density fractions by displacing the gradient medium with sterile water using a programmable syringe pump. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
|
| 发布日期 | 2025-09-27 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR2171963 |
CRR2171963_r1.fq.gz
CRR2171963_r2.fq.gz
|
5.7
5.01
|
|
| 提交者 | Wei Zhou (wei.zhou@ibcas.ac.cn) |
|---|
| 所属单位 | Institute of Botany, Chinese Academy of Sciences |
|---|
| 提交日期 | 2025-09-25 |
|---|
