| 实验编号 | CRX2077080 |
| 物种名称 | Severe acute respiratory syndrome coronavirus 2 |
| 标题 | Vero_Infected_DMSO_3hpi_Replicate1 |
| 项目编号 | PRJCA047637 |
| 样本编号 | SAMC6004211 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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To delineate the dynamic regulatory network of the viral infection-host response, we infected Huh7 (immune-competent) and Vero-E6 cells (immune-deficient30) with the SARS-CoV-2 strain. Both cell lines are highly susceptible to SARS-CoV-2 infection due to their expression of the viral entry receptor ACE2. Huh7 cells were cultured under two conditions, with and without SARS-CoV-2 infection. Vero-E6 cells were cultured under four conditions: mock control (untreated), infected by SARS-CoV-2, treated with remdesivir (RDV), and treated with both RDV and SARS-Cov-2. The total RNAs were individually extracted from each sample at 3 hours post infection (h.p.i.), 18 h.p.i. and 24 h.p.i. to simulate the early, middle and late stages of viral infection, and subjected to Poly(A) RNA enriched library constructions before deeply sequenced on Illumina Novaseq system. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
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| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2025-10-24 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
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| CRR2226211 |
CRR2226211_r1.fq.gz
CRR2226211_r2.fq.gz
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4,913.75
5,084.03
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| 提交者 | Yan Zhao (yanzhao1006@gmail.com) |
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| 所属单位 | Southern University of Science and Technology |
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| 提交日期 | 2025-10-20 |
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