| 实验编号 | CRX2185322 |
| 物种名称 | human gut metagenome |
| 标题 | ViromeMN30 |
| 项目编号 | PRJCA030971 |
| 样本编号 | SAMC6130773 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Fecal samples were processed for viral metagenomic sequencing as follows: samples were suspended in 10 mL of sterile PBS, vortexed, and centrifuged at 7000 g for 10 min. Supernatants were filtered through a 0.22-um sterile membrane and treated with 6% (w/v) polyethylene glycol 6000 and 0.2 M NaCl to precipitate particles for 2 h. After centrifugation, the pellet was resuspended in 200 uL of nuclease-free water and treated with DNase I for 10 min to remove free DNA. Viral nucleic acids were extracted using the Tiangen Viral DNA/RNA Extraction Kit according to the manufacturers protocol. Sequencing libraries were prepared with the Illumina TruSeq DNA Sample Preparation Kit, following the manufacturers protocol, and sequenced on an Illumina NovaSeq 6000 platform, generating 150-bp paired-end reads. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2025-11-21 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR2342135 |
CRR2342135_r1.fq.gz
CRR2342135_r2.fq.gz
|
3,050.32
3,097.35
|
|
| 提交者 | Ping Li (liping.8@163.com) |
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| 所属单位 | The First Medical Center of PLA General Hospital |
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| 提交日期 | 2025-11-19 |
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