| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
| CAM001070-5096 |
Genomic libraries were prepared using Illumina DNA Prep Tagmentation kit (Illumina, San Diego, CA), following the manufacturers instructions except for changes that increased library insert size to a median about 600 bp. Libraries had a final elution of 10 l Illumina resuspension buffer. Illumina-DNA/RNA UD Indexes Plate A, B, C and D dual index adapters were ordered from Integrated DNA Technologies (Coralville, IA) and used at 1 M final concentration. Instead of pooling equal volume, individual libraries were quantified using the KAPA Library Quantification Kit (Roche). Libraries were quantified in 10 l volume reactions and 90-s annealing/extension PCR, and then pooled and normalized to 4nM. Pooled libraries were re-quantified by ddPCR on a QX200 system (Bio-Rad, Hercules, CA), using the Illumina TruSeq ddPCR Library Quantification Kit and following the manufacturers protocols. Libraries were sequenced using a 2 x 250 bp paired end v2 reagent kit on a MiSeq instrument (Illumina) at 16 pM, following the manufacturers protocols. |
WGS |
GENOMIC |
RANDOM |
PAIRED
|
|
