| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
| ibm-3 |
sequencing libraries were generated and index codes were added by VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (NR604-01/02). Poly-T oligo-attached magnetic beads were used to purify mRNA from total RNA and double stranded cDNA was synthesized, with a tail attached to the end and connected to the sequencing connector. After selection of fragment size, cDNA library was produced by PCR enrichment and effective concentration was examined by Bio-RAD CFX 96 fluorescence quantitative PCR instrument. The cluster generation and sequencing were performed on Novaseq 6000 S4 Platform, using NovaSeq 6000 S4 Reagent kit V1.5 |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|
