| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
| L3 |
Total genomic DNA was extracted by using a modified 2 cetyltrimethylammonium bromide (CTAB) procedure (Doyle, 1987) from 20 mg silica gel-dried leaves. The qualified and purified DNA samples were randomly fragmented with a Covaris sonication device. The DNA fragments were end-repaired, phosphorylated, and A-tailed. Adapters were then ligated with index adapters. The ligated fragments were amplified for library construction. The qualified libraries were applied to an Illumina flowcell for cBOT cluster generation. Sequencing was performed on an Illumina MiSeq instrument. At least 3.5 gigabases (Gb) of 2 150 bp short-read data were obtained for each sample. |
WGS |
GENOMIC |
RT-PCR |
PAIRED
|
|
