| Library |
| Library name |
Construction Protocol |
Strategy |
Source |
Selection |
Layout |
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Total RNA was isolated and purified using TRIzol reagent (Invitrogen, USA) following the manufacturer's procedure. Poly (A) RNA is purified from 30 μg total RNA using Dynabeads OligodT 25-61005 (Thermo Fisher, USA) using two rounds of purification. The cleaved RNA fragments were incubated for 2h at 4 °C with m6A-specific antibody (Synaptic Systems, cat.202003, Germany) in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630). The IP RNA was reverse-transcribed to create the cDNA by SuperScript II Reverse Transcriptase (Invitrogen, cat.1896649, USA). At last, we performed sequencing using Illumina NovaSeq6000 PE150 (LC-Bio Technology Co., Ltd., Hangzhou, China). |
MeRIP-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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