| Library |
| Library name |
Construction Protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was isolated and purified using TRIzol reagent (Invitrogen, USA) following the manufacturer's procedure. Poly (A) RNA is purified from 30 μg total RNA using Dynabeads OligodT 25-61005 (Thermo Fisher, USA) using two rounds of purification. Then the cleaved RNA fragments were reverse-transcribed to create the cDNA by SuperScript II Reverse Transcriptase (Invitrogen, cat. 1896649, USA), which were next used to synthesise U-labeled second-stranded DNAs with E. coli DNA polymerase I (NEB, cat.m0209, USA), RNase H (NEB, cat.m0297, USA) and dUTP Solution (Thermo Fisher, cat.R0133, USA). At last, we performed sequencing using Illumina NovaSeq6000 PE150 (LC-Bio Technology Co., Ltd., Hangzhou, China). |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
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