Basic Information

Arachis hypogaea
Accession: ICG00006
Latin Name: Arachis hypogaea
Common Name: Peanut
Taxonomy ID: 3818
Description: Arachis hypogaea L., an allotetraploid species, is one of the Wve most important oilseed crops cultivated worldwide. It is an important food and oil crop, is grown throughout the tropics and subtropics. Cultivated peanut is an allotetraploid (AABB genome). Its ancestral species are most likely the diploid Arachis duranensis and Arachis ipaensis, which contributed the A and B subgenomes, respectively Peanut seed contains 50% oil, of which 80% consists of oleic acids and linoleic acids.

Gene Summary

mRNA

ICG00006-1
ICG00006-2

Studies (2)

ICG00006-1

Abiotic Stresses

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
ADH3 Alcohol Dehydrogenase
Salinity Stress
Drought Stress
EG529529
F: GCTTCAAGAGCAGGTCACAAGT
R: GAGACATCCTCCTTCGTGCATA
143 60 SYBR
G6PD Glyceraldehyde-3-Phosphate Dehydrogenase
Salinity Stress
Drought Stress
EG030635
F: ACCATTCCAGAGGCTTATGAGC
R: AAGGGAGTGACTTGAACTCTCC
151 60 SYBR
ELF1B Elongation Factor
Salinity Stress
Drought Stress
EE126175
F: AAGCTTCCCTGGCAAAGCTCAA
R: TTCCTCAGCTGCCTTCTTATCC
153 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Pooja Bhatnagar-Mathur
Email: p.bhatnagar@cgiar.org
Institution: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Andhra Pradesh, India

Reference

Evaluation and validation of reference genes for normalization of quantitative real-time PCR based gene expression studies in peanut.
Reddy DS, Bhatnagar-Mathur P, Cindhuri KS, Sharma KK.
PloS one. 2013-10-22

ICG00006-2

Developmental Stages
Abiotic Stresses

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
UKN1 Hypothetical Protein
Leaf
Stem
Cotyledon
Hypocotyl
Root
Salinity Stress
EG028875
F: AACGAAGACGACGACGAAGAAC
R: GAACAATAGCAACAAGAAGCAAAGC
102 60 SYBR
TUA5 Tubulin
Leaf
Stem
Cotyledon
Hypocotyl
Root
Salinity Stress
GO264294
F: CTGATGTCGCTGTGCTCTTGG
R: CTGTTGAGGTTGGTGTAGGTAGG
94 60 SYBR
UKN2 Hypothetical Protein
Leaf
Stem
Cotyledon
Hypocotyl
Root
Salinity Stress
GO334386
F: GGAAGATGGCGAGAAGCAACAG
R: GCGAAGAGCGTAGAGTAGAAGTG
89 60 SYBR
ACT11 Actin
Leaf
Stem
Cotyledon
Hypocotyl
Root
Salinity Stress
GO264911
F: TTGGAATGGGTCAGAAGGATGC
R: GCTTCTTACTGAGGCACCACT
196 60 SYBR
EF1b Elongation Factor
Low Temperature Condition
GO339841
F: GCTGCTGCTGCTGATGACG
R: TGCCTTCTTATCCTCCTCTGTCTC
66 60 SYBR
HDC Helicase
Salinity Stress
Root
EE123564
F: GCCACCAGAGCCGCTATCG
R: GCCACCAGAGCCGCTATCG
196 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Shanlin Yu
Email: yshanlin1956@163.com
Institution: Shandong Peanut Research Institute, Qingdao 266100, People's Republic of China.

Reference

Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.
Chi X, Hu R, Yang Q, Zhang X, Pan L, Chen N, Chen M, Yang Z, Wang T, He Y, Yu S.
Molecular genetics and genomics : MGG. 2011-12-28