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Brassica napus

Basic Information

Brassica napus
Accession: ICG00012
Latin Name: Brassica napus
Common Name: Oilseed rape,Rapaseed
Taxonomy ID: 3708
Description: Brassica napus is one of the most agriculturally important crops in the world, because of its large oilseed production. During cultivation of oilseed rape, it will be exposed to various adverse environmental conditions, including abiotic and biotic stresses, such as drought, high-salinity, and pathogens, which result in production losses. In recent years, more and more researchers have focused on gene expression studies in this crop under various exogenous stimulations to understand its defense mechanisms.

Gene Summary

MicroRNA

ICG00012-2
miR167-1_2 miR11-1 miR159-1 miR168-1

mRNA

ICG00012-1
ACT7 SAND UBC21 PP2A ZNF TIP41 F-box

Studies (2)

ICG00012-1

Abiotic Stresses
Biotic Stresses
Hormone Treatments
Temperature Conditions

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
ACT7 Actin
Chromium Stress
Methyl Jasmonate (Meja) Treatment
Abscisic Acid (Aba) Treatment
 Bra028615
F: GCTGACCGTATGAGCAAAG
R: AAGATGGATGGACCCGAC
 182 - SYBR
SAND Sand Family Protein
Low Temperature Condition
 Bra000494
F: GCTGAAGGTGGATTGCGTG
R: GGAGTTTCTGGTATGCTCGGTAT
 244 - SYBR
UBC21 Ubiquitin
Drought Stress
Sclerotinia Sclerotiorum Stress
 EV086936
F: CCTCTGCAGCCTCCTCAAGT
R: CATATCTCCCCTGTCTTGAAATGC
 77 - SYBR
PP2A Protein Phosphatase
Salinity Stress
Drought Stress
Methyl Jasmonate (Meja) Treatment
Sclerotinia Sclerotiorum Stress
 Bra012474
F: GTCAACAATCCGCACTACCTACA
R: CAACCACGACGGGAAGAAAC
 112 - SYBR
ZNF Zinc Finger Protein
Salicylic Acid (Sa) Treatment
 Bra032656
F: TTCAGGCGGTTTATGGC
R: TCGGGCTTTGCGTAGTT
 168 - SYBR
TIP41 Tonoplast Intrinsic Protein
Salinity Stress
Methyl Jasmonate (Meja) Treatment
Sclerotinia Sclerotiorum Stress
Salicylic Acid (Sa) Treatment
Abscisic Acid (Aba) Treatment
 Bra011516
F: TGAAGAGCAGATTGATTTGGCT
R: ACACTCCATTGTCAGCCAGTT
 101 - SYBR
F-box F-Box
Low Temperature Condition
Salicylic Acid (Sa) Treatment
 Bra006317
F: GAGATAAGTCGCTTCCTACCG
R: TGTTCCCATTGCCCTGTG
 184 - SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder

Contact

Name: Zheng Wang
Email: xltan@ujs.edu.cn
Institution: Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, People's Republic of China

Reference

Selection of reference genes for quantitative reverse-transcription polymerase chain reaction normalization in Brassica napus under various stress conditions.
Wang Z, Chen Y, Fang H, Shi H, Chen K, Zhang Z, Tan X.
Molecular genetics and genomics : MGG. 2014-04-27

ICG00012-2

Developmental Stage

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
miR167-1_2 MiRNA
Seed Development
Flower Stage
Leaf
 -
F: TGAAGCTGCCAGCATGATCTA
R: TGAAGCTGCCAGCATGATCTA
 71 60 SYBR
miR11-1 MiRNA
Seed Development
Flower Stage
Leaf
 -
F: TGCGGCAACCAAACGAACATG
R: TGCGGCAACCAAACGAACATG
 71 60 SYBR
miR159-1 MiRNA
Seed Development
Flower Stage
Leaf
 -
F: TTTGGATTGAAGGGAGCTCTA
R: TTTGGATTGAAGGGAGCTCTA
 71 60 SYBR
miR168-1 MiRNA
Seed Development
Flower Stage
Leaf
 -
F: TCGCTTGGTGCAGGTCGGGAA
R: TCGCTTGGTGCAGGTCGGGAA
 71 60 SYBR

Molecular Types:

MicroRNA

Evaluation Methods

geNorm
NormFinder

Contact

Name: Ana Paula Ko
Email: apkorbes@gmail.com
Institution: Departamento de Genetica, PPGGBM, Universidade Federal do Rio Grande do Sul, UFRGS, Av. Bento Gonc ̧alves 9500, Predio 43323 M, Sala 204/NGFP, Agronomia, Porto Alegre, RS 91501-970, Brazil

Reference

Comprehensive selection of reference genes for quantitative gene expression analysis during seed development in Brassica napus.
Machado RD, Christoff AP, Loss-Morais G, Margis-Pinheiro M, Margis R, Körbes AP.
Plant cell reports. 2015-02-27