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Pennisetum glaucum

Basic Information

Pennisetum glaucum
Accession: ICG00080
Latin Name: Pennisetum glaucum
Common Name: Pearl millet
Taxonomy ID: 4543
Description: Pennisetum glaucum is widely used small-grained C4 panicoid crop grown for forage, grain and stover with excellent nutrient composition in the arid and semi-arid regions of Asia and Africa. It is also considered a potential biofuel grain feedstock which is grown in areas frequented with one or more abiotic stresses. The superior drought and heat tolerance make it an model crop for functional genomic studies to understand the molecular basis of abiotic stress tolerance and adaptation.

Gene Summary

mRNA

ICG00080-2
EF-1A EIF4A
ICG00080-1
EF-1A UBC-E2

Studies (2)

ICG00080-1

Abiotic Stresses
Hormone Treatments
Tissues
Temperature Conditions
Developmental Stages

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
EF-1A Elongation Factor
Leaf
Stem
Sheath
Root
Booting Stage
Grain Filling Stage
Seed
Drought Stress
Salinity Stress
High Temperature Condition
Low Temperature Condition
Abscisic Acid (Aba) Treatment
 EF694165
F: GTTACAACCCAGACAAGATTGC
R: TGGACCTCTCAATCGTGTTG
 72 60 SYBR
UBC-E2 Ubiquitin-Conjugating Enzyme
Leaf
Stem
Sheath
Root
Booting Stage
Grain Filling Stage
Seed
Drought Stress
Salinity Stress
High Temperature Condition
Low Temperature Condition
Abscisic Acid (Aba) Treatment
 CD724586
F: ACCGCCTGACAATCCCTATG
R: GGGATAGTCTGGCGGAAAATG
 119 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder

Contact

Name: Radha Shivhare
Email: charulata14@gmail.com
Institution: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow-226001, India.

Reference

Selection of suitable reference genes for assessing gene expression in pearl millet under different abiotic stresses and their combinations.
Shivhare R, Lata C.
Scientific reports. 2016-03-14

ICG00080-2

Tissues
Genotypes
Hormone Treatments

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
EF-1A Elongation Factor
Seedling
Leaf
Panicle
Root
Hhb67
H77
Prlt2/89-33
Hrc1078
Hrc108
 EF694165
F: AATGATCCGCTGCTGTAACAAG
R: AGGCAATCTTGTCTGGGTTGTA
 128 59-63 SYBR
EIF4A Eukaryotic Initiation Factor
Abscisic Acid (Aba) Treatment
Drought Stress
Salinity Stress
High Temperature Condition
Low Temperature Condition
Seedling
Leaf
Panicle
Root
Hhb67
H77
Prlt2/89-33
Hrc1078
Hrc108
 EU856535
F: ATCGTGAGCTTTACATCCATCG
R: TATCCCTCAGGATACGGATGTC
 105 59-63 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Palakolanu Sudhakar Reddy
Email: p.sudhakarreddy@cgiar.org
Institution: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad 502 324, Telangana, India

Reference

Cloning and validation of reference genes for normalization of gene expression studies in pearl millet [Pennisetum glaucum (L.) R. Br.] by quantitative real-time PCR
Palakolanu Sudhakar Reddy,Dumbala Srinivas Reddy,Kiran K. Sharma,Pooja Bhatnagar-Mathur,Vincent Vadez
Plant Gene. 2015-2-18