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Solanum lycopersicum

Basic Information

Solanum lycopersicum
Accession: ICG00102
Latin Name: Solanum lycopersicum
Common Name: Tomato
Taxonomy ID: 4081
Description: Solanum lycopersicum is commonly known as a tomato plant, which belongs to the nightshade Solanaceae family. It is originated in Central and South America. The domestication of the tomato and associated selective pressures eventually led to the large-fruited varieties cultivated today. Varieties display considerable variance in fruit colour intensity, shape, and quality.

Gene Summary

mRNA

ICG00102-1
GAPDH UBI ACT UK CyP
ICG00102-2
CAC Expressed
ICG00102-5
ARD2 VIN3
ICG00102-3
ACT EF1 PP2Acs RPL2 UBI
ICG00102-4
PP2Acs

Studies (5)

ICG00102-1

Biotic Stresses
Viral Infection
Tissues

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Leaf
Root
Cmv-Fny Infection
PVY Infection
 U93208
F: ACCACAAATTGCCTTGCTCCCTTG
R: ATCAACGGTCTTCTGAGTGGCTGT
 110 60 SYBR
UBI Ubiquitin
Leaf
Root
Cmv-Fny Infection
PVY Infection
 X58253
F: TCGTAAGGAGTGCCCTAATGCTGA
R: CAATCGCCTCCAGCCTTGTTGTAA
 119 60 SYBR
ACT Actin
Leaf
Root
Cmv-Fny Infection
PVY Infection
 BT013707
F: AGGCAGGATTTGCTGGTGATGATGCT
R: ATACGCATCCTTCTGTCCCATTCCGA
 107 60 SYBR
UK Uridylate Kinase
Leaf
Root
Cmv-Fny Infection
PVY Infection
 AK322232
F: TGGTAAGGGCACCCAATGTGCTAA
R: ATCATCGTCCCATTCTCGGAACCA
 114 60 SYBR
CyP Cytochrome
Root
 AK326854
F: CTCTTCGCCGATACCACTCC
R: TCACACGGTGGAAGGTTGAG
 120 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: FABRIZIO CILLO
Email: f.cillo@ba.ivv.cnr.it
Institution: Istituto di Virologia Vegetale, CNR, Via Amendola 165/A, 70126 Bari, Italy

Reference

Evaluation of reference genes for quantitative reverse-transcription polymerase chain reaction normalization in infected tomato plants.
Mascia T, Santovito E, Gallitelli D, Cillo F.
Molecular plant pathology. 2010-11-01

ICG00102-2

Tissues
Genotypes

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
CAC Clathrin Adaptor Complex
Fruit Development
Mt-Rg1 Genotype
 -
F: CCTCCGTTGTGATGTAACTGG
R: ATTGGTGGAAAGTAACATCATCG
 - 60 SYBR
Expressed Expressed Protein
Fruit Development
Mt-Rg1 Genotype
 -
F: GCTAAGAACGCTGGACCTAATG
R: TGGGTGTGCCTTTCTGAATG
 - 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder

Contact

Name: Stefan de Folter
Email: stefan.defolter@cinvestav.mx
Institution: Unidad de Genmica Avanzada Laboratorio Nacional de Genmica para la Biodiversidad, Centro de Investigacin y de Estudios Avanzados del Instituto Politcnico Nacional, Irapuato, Mexico

Reference

Selection of Reference Genes for Quantitative Real-Time RT-PCR Studies in Tomato Fruit of the Genotype MT-Rg1.
González-Aguilera KL, Saad CF, Chávez Montes RA, Alves-Ferreira M, de Folter S.
Frontiers in plant science. 2016-09-13

ICG00102-3

Temperature Conditions
Nutrient Conditions
Photoperiod Conditions
Abiotic Stresses

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
ACT Actin
Light Stress
Low Temperature Condition
Nitrogen (N) Starvation
 TC194780
F: GAAATAGCATAAGATGGCAGACG
R: ATACCCACCATCACACCAGTAT
 159 60 SYBR
EF1 Elongation Factor
Low Temperature Condition
Nitrogen (N) Starvation
 X14449
F: GGAACTTGAGAAGGAGCCTAAG
R: CAACACCAACAGCAACAGTCT
 158 60 SYBR
PP2Acs Protein Phosphatase
Light Stress
Low Temperature Condition
Nitrogen (N) Starvation
 AY325817
F: CGATGTGTGATCTCCTATGGTC
R: AAGCTGATGGGCTCTAGAAATC
 149 60 SYBR
RPL2 Ribosomal Protein L2
Light Stress
Low Temperature Condition
Nitrogen (N) Starvation
 X64562
F: GTCATCCTTTCAGGTACAAGCA
R: CGTTACAAACAACAGCTCCTTC
 156 60 SYBR
UBI Ubiquitin
Light Stress
Low Temperature Condition
Nitrogen (N) Starvation
 TC193502
F: GGACGGACGTACTCTAGCTGAT
R: AGCTTTCGACCTCAAGGGTA
 134 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm

Contact

Name: Trond Lvdal
Email: trond.lovdal@uis.no
Institution: Faculty of Science and Technology, Centre for Organelle Research, University of Stavanger, N-4036 Stavanger, Norway

Reference

Reference gene selection for quantitative real-time PCR normalization in tomato subjected to nitrogen, cold, and light stress.
Løvdal T, Lillo C.
Analytical biochemistry. 2009-01-24

ICG00102-4

Tissues

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
PP2Acs Protein Phosphatase
Seed
 -
F: CGATGTGTGATCTCCTATGGTC
R: AAGCTGATGGGCTCTAGAAATC
 149 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder

Contact

Name: Bas J. W. Dekkers
Email: bas.dekkers@wur.nl
Institution: Wageningen Seed Lab, Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

Reference

Identification of reference genes for RT-qPCR expression analysis in Arabidopsis and tomato seeds.
Dekkers BJ, Willems L, Bassel GW, van Bolderen-Veldkamp RP, Ligterink W, Hilhorst HW, Bentsink L.
Plant & cell physiology. 2011-08-18

ICG00102-5

Bacterial Infection
Biotic Stresses

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
ARD2 N-Alpha-Acetyltransferase
P. Fluorescens Infection
P. Syringaeinfection
Non-Treated Tissue
 Solyc01g104170
F: TGTTCATCAGTGTGCTAGTG
R: GCTGTCCTTCCTTCTGAATC
 99 60 SYBR
VIN3 Vernalization Insensitive
P. Fluorescens Infection
P. Syringaeinfection
Non-Treated Tissue
 Solyc07g018270
F: ACTGGTTTGCCTGTGAAG
R: CTCAGAGAGAAGGGCATCTA
 114 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Hernan G. Rosli
Email: hrosli@agro.unlp.edu.ar
Institution: Instituto de Fisiologa Vegetal, INFIVE, Universidad Nacional de La Plata, CONICET, La Plata, Buenos Aires, Argentina

Reference

Use of RNA-seq data to identify and validate RT-qPCR reference genes for studying the tomato-Pseudomonas pathosystem.
Pombo MA, Zheng Y, Fei Z, Martin GB, Rosli HG.
Scientific reports. 2017-03-20