Basic Information

Stipa grandis
Accession: ICG00105
Latin Name: Stipa grandis
Common Name: Needlegrass
Taxonomy ID: 408131
Description: Stipa grandis P. Smirn. is a C3 perennial bunchgrass that is distributed widely in eastern Eurasian steppes and the middle Eurasian steppe zone. This species is dominant in the typical steppe of the Xilingole Plateau of Inner Mongolia. The S. grandis steppe acts as a natural green barrier and plays an important role in sandstorm prevention.

Gene Summary

mRNA

ICG00105-1

Studies (1)

ICG00105-1

Abiotic Stresses
Temperature Conditions
Hormone Treatments
Ph Conditions

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
EF1beta Elongation Factor
Drought Stress
Salinity Stress
Jasmonic Acid (Ja) Treatment
-
F: CCATCAACGAATACGTCCAGAG
R: CCCCAGTTCCAAGAATCCACTA
97 60 SYBR
UBC15 Ubiquitin
Drought Stress
Salinity Stress
High Ph Condition
Wounding Stress
Abscisic Acid (Aba) Treatment
-
F: GACCGCTATGTTAGGAACTGCC
R: AGAAGAGCCAAACCAATGCC
100 60 SYBR
ACT Actin
High Temperature Condition
-
F: GCATGAAGGTGAAGGTGGTT
R: TCATCGTACTCTGCCTTGGA
121 60 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
High Temperature Condition
-
F: GGTATGTCCTTCCGTGTTCC
R: CGCCTTGATAGCCTTCTTGA
105 60 SYBR
SKIP5 Ask-Interacting Protein
Low Temperature Condition
-
F: TGTGAGCAAAACCCTCTGGAT
R: TTGAGCGATGAGAATGAGTCATACT
80 60 SYBR
UBC17 Ubiquitin
Low Temperature Condition
Jasmonic Acid (Ja) Treatment
Salicylic Acid (Sa) Treatment
-
F: TATCCGTTCAAGCCTCCAAAG
R: GGTCGGTAAGTAGTGAGCAGATT
160 60 SYBR
HERC2 Hect Domain E3 Ubiquitin Ligases
High Ph Condition
Salicylic Acid (Sa) Treatment
-
F: ATGGGCTTCGGGAGTTTAGG
R: GCTTATTTGGGAGACATAGTGACC
105 60 SYBR
UBC2 Ubiquitin
Wounding Stress
-
F: AACATCACGGTCTGGAACGC
R: GTTGGTGGCTTGTTGGGATAA
113 60 SYBR
CUL4 Cullin
Abscisic Acid (Aba) Treatment
-
F: GTGGAAGGCGTCTGATGTGG
R: GCTTAGCTTTTGTGCGTCGTT
140 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Xiangyang Hou
Email: Houxy16@126.com
Institution: Institute of Grassland Research, Chinese Academy of Agricultural Sciences

Reference

Selection of Reference Genes for qRT-PCR Analysis of Gene Expression in Stipa grandis during Environmental Stresses.
Wan D, Wan Y, Yang Q, Zou B, Ren W, Ding Y, Wang Z, Wang R, Wang K, Hou X.
PloS one. 2017-01-05