Basic Information

Vitis vinifera
Accession: ICG00113
Latin Name: Vitis vinifera
Common Name: Common grape vine
Taxonomy ID: 29760
Description: Vitis vinifera is considered to be one of the major fruit crops in the world based on hectares cultivated and economic value. Grapes are used not only for wine but also for fresh fruit, dried fruit, and juice production. Wine is by far the major product of grapes. There are currently between 5,000 and 10,000 varieties of grapes though only a few are of commercial significance for wine and table grape production.

Gene Summary

MicroRNA

ICG00113-3

mRNA

ICG00113-2
ICG00113-1

Small nucleolar RNAs

ICG00113-3

Studies (3)

ICG00113-1

Biotic Stresses
Fungal Infection
Cultivars

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
UBQ Ubiquitin
Resistant Cultivar Regent
Downy Mildew Infection
EC922622
F: GAGGGTCGTCAGGATTTGGA
R: GCCCTGCACTTACCATCTTTAAG
75 60 SYBR
EF1a Elongation Factor
Susceptible Cultivar Trincadeira
Resistant Cultivar Regent
Downy Mildew Infection
EC959059
F: GAACTGGGTGCTTGATAGGC
R: ACCAAAATATCCGGAGTAAAAGA
164 60 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Resistant Cultivar Regent
Downy Mildew Infection
EF192466
F: TCAAGGTCAAGGACTCTAACACC
R: CCAACAACGAACATAGGAGCA
226 60 SYBR
SAND Sand Family Protein
Susceptible Cultivar Trincadeira
Downy Mildew Infection
CF405409
F: CAACATCCTTTACCCATTGACAGA
R: GCATTTGATCCACTTGCAGATAAG
76 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Filipa Monteiro
Email: fimonteiro@fc.ul.pt
Institution: Plant Systems Biology Lab, Center of Biodiversity, Functional & Integrative Genomics (BioFIG), Science Faculty of Lisbon University, Lisboa, Portugal

Reference

Reference gene selection and validation for the early responses to downy mildew infection in susceptible and resistant Vitis vinifera cultivars.
Monteiro F, Sebastiana M, Pais MS, Figueiredo A.
PloS one. 2013-09-04

ICG00113-2

Biotic Stresses
Fungal Infection
Tissues

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
VATP16 ATPase
Leaf
P. Viticola Infection
B. Cinerea Infection
LOC100248726
F: CTTCTCCTGTATGGGAGCTG
R: CCATAACAACTGGTACAATCGAC
112 60 SYBR
60SRP 60S Ribosomal Subunit
Leaf
P. Viticola Infection
B. Cinerea Infection
XM_002270599
F: ATCTACCTCAAGCTCCTAGTC
R: CAATCTTGTCCTCCTTTCCT
165 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Marielle Adrian
Email: marielle.adrian@u-bourgogne.fr
Institution: Unité Mixte de Recherche INRA 1088/CNRS 5184, Université de Bourgogne Plante-Microbe-Environnement, 17 rue Sully, BP 86510, 21065 Dijon cedex, France

Reference

Identification of reference genes suitable for qRT-PCR in grapevine and application for the study of the expression of genes involved in pterostilbene synthesis.
Gamm M, Héloir MC, Kelloniemi J, Poinssot B, Wendehenne D, Adrian M.
Molecular genetics and genomics : MGG. 2011-02-22

ICG00113-3

Abiotic Stresses
Temperature Conditions

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
U6 snRNA SnoRNA
Salt Stress
Low Temperature Condition
-
F: CCGATAAAATTGGAACGATACAGAG
R: TCGATTTGTGCGTGTCATCCT
- 60 SYBR
miR160e MiRNA
Salt Stress
Low Temperature Condition
-
F: GCGGCGGTGCCTGGCTCC
R: GTGCAGGGTCCGAGGT
- 60 SYBR
miR164a MiRNA
Salt Stress
-
F: GCGGCGGTGGAGAAGCAG
R: GTGCAGGGTCCGAGGT
- 60 SYBR
miR168 MiRNA
Drought Stress
Low Temperature Condition
-
F: GCGGCGGTCGCTTGGTGC
R: GTGCAGGGTCCGAGGT
- 60 SYBR

Molecular Types:

MicroRNA @@ SnoRNA

Evaluation Methods

Contact

Name: Shiping Wang
Email: fruit@sjtu.edu.cn
Institution: Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Reference

Selection of reference genes for miRNA qRT-PCR under abiotic stress in grapevine.
Luo M, Gao Z, Li H, Li Q, Zhang C, Xu W, Song S, Ma C, Wang S.
Scientific reports. 2018-03-13