ICG00115 Zea mays - Internal Control Genes Database (ICG)

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Zea mays

Accession: ICG00115

Latin Name: Zea mays

Common Name: Corn, Maize

Taxonomy ID: 4577

Description: Zea mays subsp. mays is a large grain plant first domesticated by indigenous peoples in southern Mexico about 10,000 years ago. The leafy stalk of the plant produces separate pollen and ovuliferous inflorescences or ears, which are fruits, yielding kernels or seeds. It is one of the most important cereal crops, also plays a growing role in industry and energy resources. However, major global abiotic stresses such as drought, heat, cold, and salinity cause huge reductions in the production every year. Additionally, hormones have been implicated in the plant response to numerous abiotic and biotic stresses, as well as developmental processes.

ICG00115-1

Abiotic Stresses

Hormone Treatments

Tissues

Temperature Conditions

Internal Control Genes

Gene Symbol	Gene Model	Application Scenario	Accession Number	Primers(5' - 3') (Forward/Reverse)	Size (bp)	Annealing Temperature	Detection
EF1a	Elongation Factor	High Temperature Condition Low Temperature Condition Salinity Stress Polyethylene Glycol (Peg) Stress Abscisic Acid (Aba) Treatment Salicylic Acid (Sa) Treatment Jasmonic Acid (Ja) Treatment Ethylene Treatment Gibberellins (Gas) Treatment Root Stem Leaf	NM_001112117	F: TGGGCCTACTGGTCTTACTACTGA R: ACATACCCACGCTTCAGATCCT	135	61.37~62.13	SYBR
A-TUB	Tubulin	High Temperature Condition Low Temperature Condition Salinity Stress Polyethylene Glycol (Peg) Stress Abscisic Acid (Aba) Treatment Salicylic Acid (Sa) Treatment Jasmonic Acid (Ja) Treatment Ethylene Treatment Gibberellins (Gas) Treatment Root Stem Leaf	NM_001111987	F: CTACCTCACGGCATCTGCTATGT R: GTCACACACACTCGACTTCACG	139	61.84~62.74	SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm

NormFinder

BestKeeper

Contact

Name: Suzhi Zhang

Email: suzhi1026@163.com

Institution: Key Laboratory of Biology and Genetic Breeding of Maize in Southwest China of Agricultural Department, Maize Research Institute, Sichuan Agricultural University, Chengdu, China

Reference

ICG00115-2

Developmental Stage

Internal Control Genes

Gene Symbol	Gene Model	Application Scenario	Accession Number	Primers(5' - 3') (Forward/Reverse)	Size (bp)	Annealing Temperature	Detection
TUB	Tubulin	Grain Development	NM_001111988	F: AGAACTGCGACTGCCTCCAAAGG R: AGATGAGCAGGGTGCCCATTC	84	60	SYBR
ACT	Actin	Grain Development	J01238	F: CATGGAGAACTGGCATCACACCTT R: CTGCGTCATTTTCTCTCTGTTGGC	118	60	SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm

NormFinder

Contact

Name: Vanessa Galli

Email: vane.galli@yahoo.com.br

Institution: Empresa Brasileira de Pesquisa Agropecuária de Clima Temperado, Rodovia BR 396, Km 78 Postal code 403, Pelotas, RS, CEP 96001-970, Brazil

Reference

ICG00115-3

Tissues

Nutrient Conditions

Internal Control Genes

Gene Symbol	Gene Model	Application Scenario	Accession Number	Primers(5' - 3') (Forward/Reverse)	Size (bp)	Annealing Temperature	Detection
CUL	Cullin	Seed Root Leaf 3-Day-Old Plant 8-Day-Old Plant 23-Day-Old Plant Hydroponic Condition	GRMZM2G166694 T04	F: GAAGAGCCGCAAAGTTATGG R: ATGGTAGAAGTGGACGCACC	274	60	SYBR
FPGS	Folylpolyglutamate Synthase	Seed Root Leaf 3-Day-Old Plant 8-Day-Old Plant 23-Day-Old Plant Hydroponic Condition	GRMZM2G393334 T01	F: ATCTCGTTGGGGATGTCTTG R: AGCACCGTTCAAATGTCTCC	132	60	SYBR
LUG	Leunig	Seed Root Leaf 3-Day-Old Plant 8-Day-Old Plant 23-Day-Old Plant Hydroponic Condition	GRMZM2G425377 T01	F: TCCAGTGCTACAGGGAAGGT R: GTTAGTTCTTGAGCCCACGC	178	60	SYBR
MEP	Membrane Protein	Seed Root Leaf 3-Day-Old Plant 8-Day-Old Plant 23-Day-Old Plant Hydroponic Condition	GRMZM2G018103 T01	F: TGTACTCGGCAATGCTCTTG R: TTTGATGCTCCAGGCTTACC	203	60	SYBR
UBCP	Ubiquitin-Conjugating Enzyme	Seed Root Leaf 3-Day-Old Plant 8-Day-Old Plant 23-Day-Old Plant Hydroponic Condition	GRMZM2G102471 T01	F: CAGGTGGGGTATTCTTGGTG R: ATGTTCGGGTGGAAAACCTT	231	60	SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm

NormFinder

BestKeeper

Contact

Name: Silvia Quaggiotti

Email: silvia.quaggiotti@unipd.it

Institution: Department of Agricultural Biotechnologies, University of Padua, 35020 Legnaro (PD), Italy

Basic Information

Gene Summary

mRNA

Studies (3)

ICG00115-1

Internal Control Genes

Molecular Types:

Evaluation Methods

Contact

Reference

ICG00115-2

Internal Control Genes

Molecular Types:

Evaluation Methods

Contact

Reference

ICG00115-3

Internal Control Genes

Molecular Types:

Evaluation Methods

Contact

Reference

ICG00115-3	CUL FPGS LUG MEP UBCP
ICG00115-2	TUB ACT
ICG00115-1	EF1a A-TUB