Basic Information

Aphis gossypii
Accession: ICG00119
Latin Name: Aphis gossypii
Common Name: Greenfly, Cotton aphid, Melon aphid
Taxonomy ID: 80765
Description: Aphis gossypii, the cotton aphid, is a tiny greenfly that sucks sap from plants. It belongs to the superfamily Aphidoidea in the order Hemiptera. It is a widely distributed pest of a variety of agriculturally important crops in the families Cucurbitaceae, Rutaceae and Malvaceae, causing damage through feeding and virus transmission. The cotton aphid control is primarily dependent on the application of insecticides, often with multiple applications each year in China.

Gene Summary

mRNA

ICG00119-1

Studies (1)

ICG00119-1

Developmental Stages
Temperature Conditions
Populations
Abiotic Stresses

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)
Size (bp) Annealing Temperature Detection
EF1 Elongation Factor
Nymph
Alate
Apterou
Xinjiang Uygur Autonomous Region
Hubei Province
Shandong Province
Henan Province
EU019874.1
F: GAAGCCTGGTATGGTTGTCGT
R: GGGTGGGTTGTTCTTTGTG
187 60 SYBR
B-ACT Actin
Nymph
Alate
Apterou
Xinjiang Uygur Autonomous Region
Hubei Province
Shandong Province
Henan Province
KF018928.1
F: TCTTGGGAATGGAATCTTGC
R: GGACAGAGAAGCCAAGATGG
254 60 SYBR
RPL7 Ribosomal Protein L7
Nymph
Alate
Apterou
High Temperature Condition
Low Temperature Condition
KP676382
F: TGCCGGAGTCTGTACTCAA
R: TCACACCACGAATACGCA
255 60 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
High Temperature Condition
Low Temperature Condition
KP676380
F: ACTACTGTTCATGCAACCACCG
R: GCTGCTTCCTTAACCTTATCCT
272 60 SYBR
18S 18S Ribosomal RNA
Feeding Assay Experiment
KF018922.1
F: ATTGACGGAAGGGCACC
R: CGCTCCACCAACTAAGAACG
157 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

Contact

Name: Xi-Wu Gao
Email: gaoxiwu@263.net.cn
Institution: Department of Entomology, China Agricultural University, Beijing 100193, People's Republic of China

Reference

Identification and Validation of Reference Genes for the Normalization of Gene Expression Data in qRT-PCR Analysis in Aphis gossypii (Hemiptera: Aphididae).
Ma KS, Li F, Liang PZ, Chen XW, Liu Y, Gao XW.
Journal of insect science (Online). 2016-02-05