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Helicoverpa armigera

Basic Information

Helicoverpa armigera
Accession: ICG00151
Latin Name: Helicoverpa armigera
Common Name: Cotton bollworm
Taxonomy ID: 29058
Description: Helicoverpa armigera, the cotton bollworm or corn ear worm, is one of the most serious agricultural pests in Asia, Africa, southern Europe and Australia. Due to heavy selection pressure over the past 40 years, it has developed resistance to organochlorine, carbamate, organophosphate and pyrethroid insecticides in many countries and has begun to show some tolerance to newer chemistries such as fipronil, chlorfenapyr, spinosad and indoxacarb. The failure of insecticides to control resistant H. armigera has been a strong incentive for the development and adoption of Bt cotton genetically engineered to withstand bollworm attack.

Gene Summary

mRNA

ICG00151-1
RPL32 RPS15 RPL27 28S

Studies (1)

ICG00151-1

Biotic Stresses
Viral Infection
Abiotic Stresses
Temperature Conditions
Tissues

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
RPL32 Ribosomal Protein L32
Adult
Larvae
Insecticide Treatment
High Temperature Condition
Low Temperature Condition
Nuclear Polyhedrosis Virus Infection
 JQ744274.1
F: CATCAATCGGATCGCTATG
R: CCATTGGGTAGCATGTGAC
 152 60 SYBR
RPS15 Ribosomal Protein S15
Adult
Larvae
Insecticide Treatment
High Temperature Condition
Low Temperature Condition
 AY818611.1
F: CTGAGGTCGATGAAACTCTC
R: CTCCATGAGTTGCTCATTG
 107 60 SYBR
RPL27 Ribosomal Protein L27
Adult
Nuclear Polyhedrosis Virus Infection
High Temperature Condition
Low Temperature Condition
 DQ875214.1
F: ACAGGTATCCCCGCAAAGTGC
R: GTCCTTGGCGCTGAACTTCTC
 155 60 SYBR
28S 28S Ribosomal RNA
Adult
Nuclear Polyhedrosis Virus Infection
High Temperature Condition
Low Temperature Condition
 GU350477.1
F: CGATAGCGAACAAGTACCGT
R: TTCGAGTTTCGCAGGTTTAC
 100 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Xiaoxia Liu
Email: liuxiaoxia611@cau.edu.cn
Institution: Department of Entomology, China Agricultural University, Beijing 100193, China

Reference

Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in Helicoverpa armigera (Lepidoptera: Noctuidae).
Zhang S, An S, Li Z, Wu F, Yang Q, Liu Y, Cao J, Zhang H, Zhang Q, Liu X.
Gene. 2014-11-18